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Upscaling the production of sterile male mosquitoes with an automated pupa sex sorter.
Gong, Jun-Tao; Mamai, Wadaka; Wang, Xiaohua; Zhu, Jian; Li, Yongjun; Liu, Julian; Tang, Qixian; Huang, Yuanhui; Zhang, Jixin; Zhou, Jiayi; Maiga, Hamidou; Somda, Nanwintoum Séverin Bimbilé; Martina, Claudia; Kotla, Simran Singh; Wallner, Thomas; Bouyer, Jérémy; Xi, Zhiyong.
Affiliation
  • Gong JT; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Mamai W; Insect Pest Control Sub-programme, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
  • Wang X; Institut de Recherche Agricole pour le Développement (IRAD), Yaoundé, Cameroun.
  • Zhu J; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Li Y; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Liu J; Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou, China.
  • Tang Q; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Huang Y; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Zhang J; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Zhou J; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Maiga H; Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China.
  • Somda NSB; Insect Pest Control Sub-programme, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
  • Martina C; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest (IRSS-DRO), Bobo-Dioulasso, Burkina Faso.
  • Kotla SS; Insect Pest Control Sub-programme, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
  • Wallner T; Unité de Formation et de Recherche en Science et Technologie (UFR/ST), Université Norbert ZONGO (UNZ), Koudougou, Burkina Faso.
  • Bouyer J; Insect Pest Control Sub-programme, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
  • Xi Z; Insect Pest Control Sub-programme, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
Sci Robot ; 9(92): eadj6261, 2024 Jul 31.
Article in En | MEDLINE | ID: mdl-39083578
ABSTRACT
Effective mosquito population suppression has been repeatedly demonstrated in field trials through the release of male mosquitoes to induce sterile mating with wild females using the incompatible insect technique (IIT), the sterile insect technique (SIT), or their combination. However, upscaling these techniques requires a highly efficient and scalable approach for the sex separation of mass-reared mosquitoes to minimize the unintentional release of females, which can lead to either population replacement or biting nuisance, a major bottleneck up to now. Here, we report the successful development of an automated mosquito pupa sex sorter that can effectively separate large numbers of males from females for population suppression of Aedes aegypti, A. albopictus, and Culex quinquefasciatus. The male production capacity of the automated sex sorter was increased by ~17-fold compared with manual sex separation with the Fay-Morlan sorter and enabled one person to separate 16 million males per week. With ~0.5% female contamination, the produced males exhibited high flight ability and mating performance. The field trial demonstrates that the quality of A. albopictus males produced using the automated sex sorter is suitable for inducing population suppression. These results indicate that the automated sex sorter offers the potential to upscale IIT and SIT against mosquito vectors for disease control.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sexual Behavior, Animal / Mosquito Control / Aedes Limits: Animals Language: En Journal: Sci Robot Year: 2024 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sexual Behavior, Animal / Mosquito Control / Aedes Limits: Animals Language: En Journal: Sci Robot Year: 2024 Document type: Article Affiliation country: China