Protocol for screening and validating antibodies specific to protein phosphorylation sites using a set of yeast biopanning approaches.
STAR Protoc
; 5(3): 103241, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39093705
ABSTRACT
Developing antibodies with high specificity against post-translationally modified epitopes remains a challenge. Yeast biopanning is well suited in screening for high-specificity binders. Here, we present a protocol for screening and validating antibodies specific to protein phosphorylation sites using a set of yeast biopanning approaches. We describe steps for screening a yeast surface display library for antibodies and other binders. We then detail procedures for validating the antibodies found by analyzing their specificity through whole-well image analysis in 96-well plates. For complete details on the use and execution of this protocol, please refer to Arbaciauskaite et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication:
United States