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Isolation, genome analysis and tissue localization of Ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia.
Gil-Ordóñez, Alejandra; Pardo, Juan M; Sheat, Samar; Xaiyavong, Khamla; Leiva, Ana M; Arinaitwe, Warren; Winter, Stephan; Newby, Jonathan; Cuellar, Wilmer J.
Affiliation
  • Gil-Ordóñez A; Cassava Program, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), The Americas Hub, Km 17, Recta Cali-Palmira, 763537, Palmira, Colombia.
  • Pardo JM; Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Calle 13 # 100-00, 760032, Cali, Colombia.
  • Sheat S; Cassava Program, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), The Americas Hub, Km 17, Recta Cali-Palmira, 763537, Palmira, Colombia.
  • Xaiyavong K; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Braunschweig, Germany.
  • Leiva AM; Cassava Program Asia Office, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), P.O. Box 783, Vientiane, Lao PDR.
  • Arinaitwe W; Cassava Program, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), The Americas Hub, Km 17, Recta Cali-Palmira, 763537, Palmira, Colombia.
  • Winter S; Cassava Program Asia Office, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), P.O. Box 783, Vientiane, Lao PDR.
  • Newby J; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Braunschweig, Germany.
  • Cuellar WJ; Cassava Program Asia Office, Crops for Nutrition and Health, International Center for Tropical Agriculture (CIAT), P.O. Box 783, Vientiane, Lao PDR.
Sci Rep ; 14(1): 18139, 2024 08 05.
Article in En | MEDLINE | ID: mdl-39103398
ABSTRACT
In Southeast Asia (SEA) fastidious fungi of the Ceratobasidium genus are associated with proliferation of sprouts and vascular necrosis in cacao and cassava, crops that were introduced from the tropical Americas to this region. Here, we report the isolation and in vitro culture of a Ceratobasidium sp. isolated from cassava with symptoms of witches' broom disease (CWBD), a devastating disease of this crop in SEA. The genome characterization using a hybrid assembly strategy identifies the fungus as an isolate of the species C. theobromae, the causal agent of vascular streak dieback of cacao in SEA. Both fungi have a genome size > 31 Mb (G+C content 49%), share > 98% nucleotide identity of the Internal Transcribed Spacer (ITS) and > 94% in genes used for species-level identification. Using RNAscope® we traced the pathogen and confirmed its irregular distribution in the xylem and epidermis along the cassava stem, which explains the obtention of healthy planting material from symptom-free parts of a diseased plant. These results are essential for understanding the epidemiology of CWBD, as a basis for disease management including measures to prevent further spread and minimize the risk of introducing C. theobromae via long-distance movement of cassava materials to Africa and the Americas.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Manihot / Genome, Fungal Country/Region as subject: Asia Language: En Journal: Sci Rep Year: 2024 Document type: Article Affiliation country: Colombia Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Manihot / Genome, Fungal Country/Region as subject: Asia Language: En Journal: Sci Rep Year: 2024 Document type: Article Affiliation country: Colombia Country of publication: United kingdom