Towards an optimized model of food allergy in zebrafish.
Mol Immunol
; 173: 110-116, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-39106608
ABSTRACT
BACKGROUND:
The prevalence of food allergies is on the rise, posing a significant challenge to public health. Rodents serve as the predominant animal model in food allergy research; yet, the application of rodent models proves to be a laborious and time-consuming endeavor. It is imperative to develop novel in vivo models.METHODS:
Ovalbumin (OVA) was administered as the allergen, following the recommended dosage used in other species. During the sensitization phase, a dosage of 0.25â¯mg per 10 tails per 1â¯L was administered twice daily, and during the challenge phase, the dosage was increased to 3 times the initial level. The study explored two dimensions of sensitization the mode of exposure, which can be either continuous or intermittent, and the duration of exposure, which includes 3 days, 5 days, and 7 days. We examined midgut pathological changes, immunoglobulins contents, and mRNA expressions associated to T helper cells (Th) 2 cytokines following exposure.RESULTS:
A significant 109.3â¯% increase in the number of eosinophils was observed in the midgut histopathology following intermittent 5-day OVA exposure, which emerged as the most effective model. OVA exposure increased concentrations of immunoglobulin M (IgM) (105.2â¯%), IgZ (312.1â¯%), and IgD (304.3â¯%) in this model. The mRNA expressions of Th2-related interleukin (IL)-4 and IL-13 were also elevated by 132.8â¯% and 421.0â¯%, respectively.CONCLUSION:
The intermittent 5-day OVA exposure was suggested to be the best constructed zebrafish food allergy model, which may be a potential tool for research into food allergies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zebrafish
/
Ovalbumin
/
Disease Models, Animal
/
Food Hypersensitivity
Limits:
Animals
Language:
En
Journal:
Mol Immunol
Year:
2024
Document type:
Article
Country of publication:
United kingdom