Your browser doesn't support javascript.
loading
Immunological Profiling of CD8+ and CD8- NK Cell Subpopulations and Immune Checkpoint Alterations in Early-Onset Preeclampsia and Healthy Pregnancy.
Szereday, Laszlo; Nagy, David U; Vastag, Fanni; Mezosi, Livia; Meggyes, Matyas.
Affiliation
  • Szereday L; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 12 Szigeti Street, 7624 Pécs, Hungary.
  • Nagy DU; Janos Szentagothai Research Centre, 20 Ifjusag Street, 7624 Pécs, Hungary.
  • Vastag F; Institute of Geobotany/Plant Ecology, Martin-Luther-University, Große Steinstraße 79/80, D-06108 Halle (Saale), Germany.
  • Mezosi L; Department of Obstetrics and Gynaecology, Medical School, University of Pécs, 7624 Pécs, Hungary.
  • Meggyes M; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 12 Szigeti Street, 7624 Pécs, Hungary.
Int J Mol Sci ; 25(15)2024 Jul 31.
Article in En | MEDLINE | ID: mdl-39125946
ABSTRACT
Despite the numerous studies on the clinical aspects of early-onset preeclampsia, our understanding of the immunological consequences of inadequate placenta development remains incomplete. The Th1-predominance characteristic of early-onset preeclampsia significantly impacts maternal immunotolerance, and the role of immune checkpoint molecules in these mechanisms is yet to be fully elucidated. Our study aims to fill these crucial knowledge gaps. A total of 34 pregnant women diagnosed with early-onset preeclampsia and 34 healthy pregnant women were enrolled in this study. A mononuclear cell fragment from the venous blood was separated and frozen. The CD8+ and CD8- NK cell subpopulations were identified and compared to their immune checkpoint molecule expressions using multicolor flow cytometry. The serum CD226 levels were measured by ELISA. Based on our measures, the frequency of the CD8- subpopulation was significantly higher than that of the CD8+ counterpart in both the NKdim and NKbright subsets. Significantly lower CD226 surface expressions were detected in the preeclamptic group compared to healthy women in all the investigated subpopulations. However, while no difference was observed in the level of the soluble CD226 molecule between the two groups, the CD112 and CD155 surface expressions were significantly different. Our study's findings underscore the significant role of the CD8+ and CD8- NK subpopulations in the Th1-dominated immune environment. This deepens our understanding of early-onset preeclampsia and suggests that each subpopulation could contribute to the compensation mechanisms and the restoration of the immunological balance in this condition, a crucial step toward developing effective interventions.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pre-Eclampsia / Killer Cells, Natural / CD8-Positive T-Lymphocytes Limits: Adult / Female / Humans / Pregnancy Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: Hungary

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pre-Eclampsia / Killer Cells, Natural / CD8-Positive T-Lymphocytes Limits: Adult / Female / Humans / Pregnancy Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: Hungary