Visualization and Quantification of Rapid Chromosome Movements at Early Stages of Mouse Meiosis.
Methods Mol Biol
; 2818: 171-177, 2024.
Article
in En
| MEDLINE
| ID: mdl-39126474
ABSTRACT
Telomere-led rapid chromosome movements (RPMs) are a conserved characteristic of chromosome dynamics in meiosis. RPMs have been suggested to influence critical meiotic functions such as DNA repair and the association of the homologous chromosomes. Here, we describe a method using 3D time-lapse fluorescence imaging to monitor RPMs in Hoechst-stained mouse seminiferous tubules explants. We supplement visualization with customized quantitative motion analysis and in silico simulation. The ability to carry out live imaging, combined with quantitative image analysis, offers a sensitive tool to investigate the regulation of RPMs, chromosome reorganizations that precede dynamic mid-prophase events, and their contribution to faithful transmission of genetic information.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Meiosis
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
United States
Country of publication:
United States