MAPK3-MYB36-ARF1 module regulates the tanshinone formation in Salvia miltiorrhiza.
Plant Signal Behav
; 19(1): 2391659, 2024 Dec 31.
Article
in En
| MEDLINE
| ID: mdl-39145499
ABSTRACT
Salvia miltiorrhiza, known as Danshen, is a traditional Chinese medicinal plant with significant cardiovascular benefits, attributed to its secondary metabolites, particularly tanshinones. Despite their medicinal value, tanshinones occur in low natural abundance, necessitating research to increase their content. This study explores the role of the ARF transcription factor (SmARF1) in tanshinone accumulation in Danshen. Overexpressing SmARF1 in hairy roots significantly increased tanshinone levels. EMSA and Dual-LUC assays revealed that SmMYB36, a transcription factor interacting with SmMAPK3, binds to and regulates the SmARF1 promoter. SmMYB36 alone inhibited the expression of SmARF1 gene, while its interaction with SmMAPK3 enhanced SmARF1 promoter activity. This MAPK3-MYB36-ARF1 module elucidates a complex regulatory mechanism for tanshinone biosynthesis, offering insights for targeted enhancement of tanshinone content through advanced biotechnological approaches.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Gene Expression Regulation, Plant
/
Salvia miltiorrhiza
/
Abietanes
Language:
En
Journal:
Plant Signal Behav
Journal subject:
BOTANICA
/
FISIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United States