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Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization.
Priyadarshani, Priyanka; Van Grouw, Alexandria; Liversage, Adrian Ross; Rui, Kejie; Nikitina, Arina; Tehrani, Kayvan Forouhesh; Aggarwal, Bhavay; Stice, Steven L; Sinha, Saurabh; Kemp, Melissa L; Fernández, Facundo M; Mortensen, Luke J.
Affiliation
  • Priyadarshani P; School of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA.
  • Van Grouw A; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Liversage AR; School of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA.
  • Rui K; School of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA.
  • Nikitina A; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Tehrani KF; Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Champaign, IL 61820, USA.
  • Aggarwal B; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USA.
  • Stice SL; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA.
  • Sinha S; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USA.
  • Kemp ML; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USA.
  • Fernández FM; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Mortensen LJ; School of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA. Electronic address: luke.mortensen@uga.edu.
Cell Rep ; 43(8): 114579, 2024 Aug 27.
Article in En | MEDLINE | ID: mdl-39153198
ABSTRACT
Mesenchymal stem/stromal cell (MSC) therapies have had limited success so far in clinical trials due in part to heterogeneity in immune-responsive phenotypes. Therefore, techniques to characterize these properties of MSCs are needed during biomanufacturing. Imaging cell shape, or morphology, has been found to be associated with MSC immune responsivity-but a direct relationship between single-cell morphology and function has not been established. We used label-free differential phase contrast imaging and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to evaluate single-cell morphology and explore relationships with lipid metabolic immune response. In interferon gamma (IFN-γ)-stimulated MSCs, we found higher lipid abundances from the ceramide-1-phosphate (C1P), phosphatidylcholine (PC), LysoPC, and triglyceride (TAG) families that are involved in cell immune function. Furthermore, we identified differences in lipid signatures in morphologically defined MSC subpopulations. The use of single-cell optical imaging coupled with single-cell spatial lipidomics could assist in optimizing the MSC production process and improve mechanistic understanding of manufacturing process effects on MSC immune activity and heterogeneity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesenchymal Stem Cells / Lipidomics Limits: Humans Language: En Journal: Cell Rep Year: 2024 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesenchymal Stem Cells / Lipidomics Limits: Humans Language: En Journal: Cell Rep Year: 2024 Document type: Article Affiliation country: United States Country of publication: United States