Protocol for the derivation of primary cancer stem cell lines from human ependymal tumors.
STAR Protoc
; 5(3): 103260, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39153201
ABSTRACT
Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines. For complete details on the use and execution of this protocol, please refer to Michealraj et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neoplastic Stem Cells
Limits:
Humans
Language:
En
Journal:
STAR Protoc
/
STAR protocols
Year:
2024
Document type:
Article
Country of publication:
United States