New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish.
Microorganisms
; 12(8)2024 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-39203417
ABSTRACT
The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established â3)-α-L-Rhap-(1â3)-α-D-ManpNAc-(1â2)-ß-D-Glcp-(1â3)-α-L-Rhap2OAc4OAc-(1â3)-ß-D-ManpNAc-(1â3)-α-D-Glcp-(1â. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Microorganisms
Year:
2024
Document type:
Article
Affiliation country:
Poland
Country of publication:
Switzerland