Smartphone-assisted colorimetric biosensor for the rapid visual detection of natural antioxidants in food samples.
Food Chem
; 462: 141026, 2025 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-39216373
ABSTRACT
Quantitative monitoring of the concentrations of epigallocatechin gallate (EGCG) and cysteine (Cys) is of great significance for promoting human health. In this study, iron/aluminum bimetallic MOF material MIL-53 (Fe, Al) was rapidly prepared under room temperature using a co-precipitation method, followed by investigating the peroxidase-like (POD-like) activity of MIL-53(Fe, Al) using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic substrate. The results showed that the Michaelis -Menten constants of TMB and H2O2 as substrates were 0.167 mM and 0.108 mM, respectively. A colorimetric sensing platform for detecting EGCG and Cys was developed and successfully applied for analysis and quantitative detection using a smartphone. The linear detection range for EGCG was 15â¼80 µM (R2=0.994) and for Cys was 7â¼95 µM (R2=0.998). The limits of detection (LOD) were 0.719 µM and 0.363 µM for EGCG and Cys, respectively. This work provides a new and cost-effective approach for the real-time analysis of catechins and amino acids.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Catechin
/
Colorimetry
/
Smartphone
/
Antioxidants
Language:
En
Journal:
Food Chem
/
Food chem
/
Food chemistry
Year:
2025
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom