Cleavage and polyadenylation factors are potential regulators of adipogenesis.
BMC Res Notes
; 17(1): 242, 2024 Sep 02.
Article
in En
| MEDLINE
| ID: mdl-39223634
ABSTRACT
OBJECTIVE:
Alternative polyadenylation (APA) is a co-transcriptional process that leads to isoform diversity in the 3' ends of mRNAs. APA is known to occur during differentiation, and its dysregulation is observed in diseases like cancer and autoimmune disorders. It has been previously reported that differentiation of 3T3-L1 cells to adipocytes leads to an overall lengthening of mRNAs, but the proteins involved in this regulation have not been identified. The expression levels of subunits of the cleavage and polyadenylation (C/P) complex can regulate the choice of poly(A) site, which in turn can affect different cellular activities. In this paper, we studied the change in levels of C/P proteins during 3T3-L1 differentiation.RESULTS:
We observed that while the RNA expression of these proteins is unchanged during differentiation, the protein levels of some subunits do change, including a decrease in levels of CPSF73, the nuclease that cuts at the poly(A) site. However, overexpression of CPSF73 alone does not affect the efficiency and rate of differentiation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
3T3-L1 Cells
/
Adipogenesis
Limits:
Animals
Language:
En
Journal:
BCM res. notes
/
BMC Res Notes
/
BMC research notes
Year:
2024
Document type:
Article
Affiliation country:
United States
Country of publication:
United kingdom