Advanced glycation end products impair the repair of injured tendon: a study in rats.
BMC Musculoskelet Disord
; 25(1): 700, 2024 Sep 03.
Article
in En
| MEDLINE
| ID: mdl-39227794
ABSTRACT
BACKGROUND:
The AGEs levels in tissues of diabetics and elderly tend to be higher than in normal individuals. This study aims to determine the effects of AGEs on Achilles tendon repair. MATERIALS ANDMETHODS:
Thirty-six male eight-week-old Sprague Dawley rats were selected in this study. The rats were randomly divided into two experimental groups and a control group after the transection of the Achilles tendon. During the tendon repair, the experimental groups were injected around the Achilles tendon with 350mmol/L (low dose group) and 1000mmol/L (high dose group) D-ribose 0.2 ml respectively to increase the AGEs level, while in the control group were given the same amount of PBS. The injections were given twice a week for six weeks. Collagen-I, TNF-α, and IL-6 expression in the healed Achilles tendon was assessed. Additionally, macroscopic, pathological, and biomechanical evaluations of Achilles tendon repair were conducted.RESULTS:
The repaired Achilles tendons in the high dose group showed severe swelling and distinctive adhesions. The histological score went up with the increase of the AGEs in the Achilles tendon (p<0.001). TNF- α and IL-6 in the Achilles tendon increased (p<0.001, p<0.001), and the production of collagen-I decreased with the accumulation of AGEs in the repaired Achilles tendon (p<0.001). The tensile strength of Achilles tendon in the high dose group was impaired significantly.CONCLUSION:
In current study, the compromised tendon repair model induced by AGEs was successfully established in rat. The study demonstrated that AGEs significantly impair Achilles tendon repair.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Achilles Tendon
/
Tendon Injuries
/
Wound Healing
/
Rats, Sprague-Dawley
/
Glycation End Products, Advanced
Limits:
Animals
Language:
En
Journal:
BMC Musculoskelet Disord
Journal subject:
FISIOLOGIA
/
ORTOPEDIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom