Anisomycin alleviates cognitive impairments and pathological features in 3xTg-AD mice.
Neuropharmacology
; 261: 110159, 2024 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-39303856
ABSTRACT
Neuroinflammation plays a pivotal role in the pathogenesis of Alzheimer's disease (AD). Anisomycin is a pyrrolidine antibiotic isolated from Streptomyces griseolus, which is an efficient anti-inflammatory agent that functions both in vivo and in vitro. However, it is not clear whether anisomycin can exert neuroprotective effect in AD. In the present study, anisomycin was intragastrically administrated to female triple-transgenic AD (3xTg-AD) model mice, then Morris water maze test was used to observe the long-term spatial memory of mice, the in vivo hippocampal field potential recording was performed to evaluate the synaptic plasticity, the Western blot and immunofluorescence were employed to detect pathological changes, and the bioinformatics analysis was used to predict the potential target of anisomycin exerting effects in AD. The results showed that anisomycin ameliorated the long-term spatial memory deficits, improved LTP depression and increased the expression of PSD-95, reduced the Aß and tau pathologies, and alleviated the activation of microglia and astrocytes in the brains of 3xTg-AD mice. In addition, the results from bioinformatics analysis showed that the potential target of anisomycin focused on inflammatory pathway. These results indicated that anisomycin exerts neuroprotective effects in 3xTg-AD mice by alleviating neuroinflammation, but the potential mechanism of anisomycin exerting neuroprotective effects needs to be further investigated.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mice, Transgenic
/
Neuroprotective Agents
/
Alzheimer Disease
/
Cognitive Dysfunction
/
Anisomycin
Limits:
Animals
Language:
En
Journal:
Neuropharmacology
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom