Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca2+-CaM signaling pathway.
Clinics (Sao Paulo)
; 79: 100485, 2024.
Article
in En
| MEDLINE
| ID: mdl-39316895
ABSTRACT
OBJECTIVE:
In the context of postoperative anal pain, understanding the intricate mechanisms and effective interventions is paramount. This study investigates the role of Muscarinic Acetylcholine Receptors (mAChRs) and the IP3-Ca2+-CaM signaling pathway in a rat model of postoperative anal pain, exploring the potential analgesic effects of electroacupuncture.METHODS:
Comprehensive approaches involving mechanical sensitivity assays, Western blotting, immunohistochemistry, and intracellular calcium concentration measurement were used.RESULTS:
The authors found elevated mAChRs expression in the postoperative pain model. Antagonizing mAChRs reduced pain sensitivity and attenuated the IP3-Ca2+-CaM pathway. Remarkably, electroacupuncture treatment further mitigated pain, potentially by suppressing this signaling cascade.INTERPRETATION:
These findings reveal a novel connection between mAChRs and the IP3-Ca2+-CaM pathway in postoperative anal pain and suggest electroacupuncture as a promising avenue for pain relief through these mechanisms, offering insights into innovative strategies for postoperative pain management.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pain, Postoperative
/
Signal Transduction
/
Electroacupuncture
/
Receptors, Muscarinic
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Rats, Sprague-Dawley
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Hemorrhoidectomy
Limits:
Animals
Language:
En
Journal:
Clinics (Sao Paulo)
Journal subject:
MEDICINA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United States