Environmental DNA reveals the spatiotemporal distribution and migration characteristics of the Yangtze Finless Porpoise, the sole aquatic mammal in the Yangtze River.

ABSTRACT

The Yangtze Finless Porpoise (YFP) is one of the 13 global flagship species identified by the World Wildlife Fund and is classified as "Critically Endangered." It is also the only extant aquatic mammal in the Yangtze River. In this study, 44 sampling points were deployed across the middle and lower reaches of the Yangtze River, with vertical sampling sections established in four key areas. Using environmental DNA (eDNA) and species distribution model(SDM), we explored the spatiotemporal distribution of YFPs and predicted their potential suitable habitats. The results indicate that the YFP has a relatively wide distribution during the flood season but exhibits clustering behavior during the dry season, showing a patchy distribution and a migratory trend from the midstream to downstream of the main channel. Predictions using the MAXENT model reveal varying trends in suitable habitat under different scenarios. Overall, YFP's potential habitat is expected to expand by 2050, but due to rising temperatures, it will contract by 2070. Elevation (dem, 65.4%), human footprint index (hfp, 8.8%), and isothermality (bio3, 8%) are key factors influencing habitat suitability. These findings demonstrate that eDNA is an effective tool for monitoring large aquatic organisms and provide scientific evidence for the conservation of the YFP.