Expression Level of Cardiac Ryanodine Receptors Dictates Properties of Cardiac Ca2+-Induced Ca2+ Release.

ABSTRACT

The type 2 ryanodine receptor (RyR2) is the major Ca2+ release channel required for Ca2+-induced Ca2+ release (CICR) and cardiac excitation-contraction coupling. The cluster organization of RyR2 at the dyad is critical for efficient CICR. Despite its central role in cardiac Ca2+ signaling, the mechanisms that control CICR are not fully understood. As a single RyR2 Ca2+ flux dictates local CICR that underlies Ca2+ spark, RyR2 density in a cluster and therefore the distance between RyR2s should have a profound impact on local CICR. Here, we studied the effect of RyR2 expression level ([RyR2]) on CICR activation, termination, and amplitude. The ER-targeted Ca2+ sensor RCEPIA-1er was used to directly measure the endoplasmic reticulum (ER) [Ca2+] (Ca2+]ER) in T-Rex-293 SERCA2a stable cell line expressing human RyR2. Cells co-expressing RyR2 and SERCA2a produced periodic [Ca2+]ER depletions in the form of spontaneous Ca2+ waves due to propagating CICR. For each studied cell, [Ca2+]ER at which Ca2+ waves are activated and terminated was analyzed as a function of [RyR2]. CICR parameters, such as [Ca2+]ER activation, termination, and amplitude, were inversely proportional to [RyR2] at low-intermediate levels. Increasing sensitivity of RyR2 to cytosolic Ca2+ lowered [Ca2+]ER at which CICR is activated and terminated. Decreasing the sensitivity of RyR2 to cytosolic Ca2+ had the opposite effect on CICR. These results suggest that RyR2 density in the release cluster should have a significant impact on local CICR activation and termination. Since SR Ca2+ load is evenly distributed throughout the SR network, clusters with higher RyR2 density would have a higher probability to initiate spontaneous CICR.