Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants.

Budowle, B; Eberhardt, P

Budowle, B; Eberhardt, P.

Hemoglobin ; 10(2): 161-72, 1986.

Article in En | MEDLINE | ID: mdl-3957695

ABSTRACT

ABSTRACT

An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.

Subject(s)

Blood Protein Electrophoresis/methods; Hemoglobinometry/methods; Hemoglobins, Abnormal/analysis; Isoelectric Focusing/methods; Electrophoresis, Polyacrylamide Gel/methods; Ethnicity; Forensic Medicine/methods; Humans; Mass Screening; Racial Groups

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Collection: 01-internacional Database: MEDLINE Main subject: Blood Protein Electrophoresis / Hemoglobins, Abnormal / Hemoglobinometry / Isoelectric Focusing Type of study: Diagnostic_studies / Screening_studies Aspects: Equity_inequality Limits: Humans Language: En Journal: Hemoglobin Year: 1986 Document type: Article Publication country: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM

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