[Photo-dependent conversions of cytochrome f in bean chloroplasts and leaves treated with polyene antibiotics]. / Fotozavisimye prevrashcheniia tsitokhroma f v khloroplastakh i list'iakh bobov, obrabotannykh polienovymi antibiotikami.
Biokhimiia
; 44(11): 2005-12, 1979 Nov.
Article
in Ru
| MEDLINE
| ID: mdl-546443
ABSTRACT
The photo-dependent absorption changes of cytochrome f in bean chloroplasts and native leaves treated with the polyene antibiotics surgumycin and filipin were studied. Upon incubation of the chloroplasts or leaves with the antibiotics the value of the photo-induced signal of cytochrome f decreased considerably; however, the kinetics of the cytochrome oxidation under the effect of the exciting light and dark reduction remained unchanged. An addition of plastocyanin to the suspension of the antibiotic-treated chloroplasts, which contained no artificial donors and acceptors, only slightly increased the absolute value of the photo-induced signal of cytochrome f. An addition of plastocyanin to the chloroplasts containing the dichlorophenolindophenol-ascorbate-methylviologen system, sharply changed the kinetics of the cytochrome f photoconversions. A simultaneous registration of the photo-induced signal of cytochrome f and the photochemical activity of photosystem I of the antibiotic-treated chloroplasts revealed differences in the degree of inhibition of the photosystem I activity and decrease of the absolute value of the cytochrome f signal. The data obtained are discussed in terms of possible alternative pathways of electron transfer in the part of the electron transporting chain under study.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyenes
/
Chloroplasts
/
Filipin
/
Cytochromes
/
Anti-Bacterial Agents
Language:
Ru
Journal:
Biokhimiia
Year:
1979
Document type:
Article