Partial purification of a serum factor that causes necrosis of tumors.
Proc Natl Acad Sci U S A
; 73(2): 381-5, 1976 Feb.
Article
in En
| MEDLINE
| ID: mdl-54919
ABSTRACT
Tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial endotoxin. Sera from normal mice, endotoxin-treated mice, and mice infected with bacillus Calmette-Guérin do not contain this factor. A 20- to 30-fold purification of the serum factor has been achieved by (NH4)2SO4 fractionation, Sephadex G-100 and G-200 gel filtration, and preparative polyacrylamide electrophoresis. Tumor necrosis factor is not bacterial endotoxin. It migrates with alpha-globulins, is made up of at least four subunits, and has a molecular weight of about 150,000. The active factor is a glycoprotein that contains sialic acid and galactosamine.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
BCG Vaccine
/
Blood Proteins
/
Glycoproteins
/
Endotoxins
/
Mycobacterium bovis
/
Neoplasms, Experimental
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
1976
Document type:
Article