Discrimination and consistency of five myosin ATPase stains in human normal and Duchenne dystrophic muscle.
Histochemistry
; 75(4): 557-71, 1982.
Article
in En
| MEDLINE
| ID: mdl-6184341
ABSTRACT
Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin. ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staining and Labeling
/
Myosins
/
Adenosine Triphosphatases
/
Muscles
/
Muscular Dystrophies
Type of study:
Prognostic_studies
Limits:
Adult
/
Child, preschool
/
Humans
/
Male
Language:
En
Journal:
Histochemistry
Year:
1982
Document type:
Article