Isolation of surface tubules of fowlpox virus.
Avian Dis
; 25(2): 454-62, 1981.
Article
in En
| MEDLINE
| ID: mdl-6266391
ABSTRACT
Surface tubules of fowlpox virus were isolated using chemical and physical methods. Suspensions of lipid cytoplasmic inclusion bodies were obtained by treating infected chorioallantoic membranes with 1% trypsin. Inclusions were treated with ultrasonic sound, detergents, and enzymes and were examined by electron microscopy. Although lipase treatment altered the morphology of lipid inclusions, no viral surface tubules were recovered. Treatment with the detergent Nonidet-P40 followed by 2-mercaptoethanol disrupted virions without allowing surface tubules to be recovered. Disruption of lipid inclusions by ultrasonic sound or manual grinding of chorioallantoic membranes produced free virions but only small numbers of tubules. These results indicate that surface tubules can be recovered, but that the lipid nature of cytoplasmic inclusions interferes with procedures commonly used in tubule purification.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Poxviridae
/
Fowlpox virus
/
Inclusion Bodies, Viral
/
Microtubules
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
Avian Dis
Year:
1981
Document type:
Article