Glutamyl-tRNA synthetases from wheat. Isolation and characterization of three dimeric enzymes.
Eur J Biochem
; 135(3): 471-7, 1983 Oct 03.
Article
in En
| MEDLINE
| ID: mdl-6617644
ABSTRACT
Three dimeric glutamyl-tRNA synthetases (GluRS) were isolated from extracts of quiescent wheat germ and wheat chloroplasts. The chloroplast enzyme (Mr = 110 000), called GluRS C, exhibits a prokaryotic (Escherichia coli) tRNA specificity. Two enzymes were found in the quiescent germ and were separated on phosphocellulose P11 one called GluRS P, probably the mitochondrial enzyme, has the same tRNA specificity as GluRS C; the other, called GluRS E, has eukaryotic (wheat germ) tRNA specificity. Both enzymes exhibit a molecular weight close to 160 000. Each of these enzymes co-eluate on hydroxyapatite and phosphocellulose chromatographies with an unstable active monomer whose molecular weight is approximately half that of the corresponding dimer. Two assumptions are discussed about these monomers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Triticum
/
Amino Acyl-tRNA Synthetases
/
Glutamate-tRNA Ligase
Language:
En
Journal:
Eur J Biochem
Year:
1983
Document type:
Article