Early chick embryonic cells can form clones in agarose cultures.
Exp Cell Res
; 152(1): 148-53, 1984 May.
Article
in En
| MEDLINE
| ID: mdl-6714316
ABSTRACT
Early chick embryonic cells prior to the formation of the primitive streak, have been cultured in a two-layer soft-agarose system. Single, primary cells when grown in this system were capable of producing colonies ranging in size from 30 to 100 cells. The plating efficiency varied between 1 and 5% and the colonies remained viable for about 2 weeks. We believe this is the first report of normal, non-passaged cells which show anchorage-independent growth properties by forming colonies in a standard agarose culture in the absence of additional factors. The importance of being able to use normal monoclonal embryonic cell populations in studying early developmental processes is also discussed.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chick Embryo
Limits:
Animals
Language:
En
Journal:
Exp Cell Res
Year:
1984
Document type:
Article