The internalisation of [125I] insulin by isolated rat hepatocytes.
Horm Metab Res
; 14(10): 536-9, 1982 Oct.
Article
in En
| MEDLINE
| ID: mdl-6757087
ABSTRACT
This work was undertaken to examine the relationship between the binding, internalisation and degradation of insulin by isolated rat hepatocytes. Internalisation of hormone reached a maximum after 5-7 minutes at 37 degrees C; at an insulin concentration of 0.1 nM, 25% of the specifically bound hormone was internalised. Internalisation and the degradation of internalised insulin were inhibited by the intralysosomal protease inhibitors chloroquine and methylamine but not by the thiol oxidant diamide. It is concluded that internalisation of insulin by rat hepatocytes is not a necessary step in the degradation of the hormone molecule.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Insulin
/
Liver
Limits:
Animals
Language:
En
Journal:
Horm Metab Res
Year:
1982
Document type:
Article