Ethidium bromide: pharmacokinetics and efficacy against trypanosme infections in rabbits and calves.
Parasitology
; 85 (Pt 1): 127-48, 1982 Aug.
Article
in En
| MEDLINE
| ID: mdl-7122121
ABSTRACT
[14C]ethidium bromide has been used to determine drug levels in tissues and body fluids of rabbits and calves following intramuscular injection. Uninfected and Trypanosoma brucei- or Trypanosoma congolense-infected animals were studied. Blood and tissue fluid level reached a maximum with 1 h and then fell rapidly; after 96 h 80-90% of the radioactivity injected had been excreted, approximately one third in urine and two thirds in faeces. By 1 h after injection of 1 mg [14C]ethidium/kg into a T. congolense-infected calf, 70-80% of the radioactivity in blood was found to be bound to trypanosomes. Doses of 1 or 10 mg/kg were found not to be curative for T congolense or T. brucei infections in rabbits drug treatment resulted in a period of sub-patent parasitaemia which was always followed by a relapse. Examination of the prophylactic action of ethidium in rabbits showed that the drug extended the pre-patent period following trypanosome inoculation but provided no absolute protection. A period of "apparent' prophylaxis observed after drug treatment of infected rabbits has been correlated with the presence of anti-trypanosome IgG in the serum.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosomiasis, African
/
Trypanosomiasis, Bovine
/
Ethidium
Limits:
Animals
Language:
En
Journal:
Parasitology
Year:
1982
Document type:
Article