Isolation and partial characterization of pancreatic polypeptide-like material in the brain of the blowfly Calliphora vomitoria.
Biochem J
; 197(3): 767-70, 1981 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-7325987
ABSTRACT
Using 10(6) flies (5 kg of heads) a pancreatic polypeptide-like material has been partially purified from the blowfly Calliphora vomitoria. The isolated material was eluted on Sephadex G-50 similarly to bovine pancreatic polypeptide and had an RF on polyacrylamide-gel electrophoresis that was identical with that of the bovine hormone. The material diluted linearly and showed parallelism with bovine standards in a bovine pancreatic polypeptide immunoassay. In specificity controls the immunoreactivity was not abolished by trasylol and no cross-reactivity was discerned in assay for glucagon, proangiotensin and cyclic AMP. These data suggest that the pancreatic polypeptide material in the brain of the blowfly has close structural similarity to the mammalian hormone.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pancreatic Polypeptide
/
Diptera
Limits:
Animals
Language:
En
Journal:
Biochem J
Year:
1981
Document type:
Article