Compaction assay: a rapid and simple in vitro method to assess the responsiveness of a biopolymer matrix to enzymatic modification.
Biomaterials
; 16(7): 553-8, 1995 May.
Article
in En
| MEDLINE
| ID: mdl-7492720
ABSTRACT
A rapid and simple in vitro method is described which measures the extent of unrecoiled solids compression when a complex biopolymer is subjected to a centrifugal force. This method, termed the compaction assay, was used to assess the response of purulent cystic fibrosis (CF) sputum samples to the addition of recombinant human deoxyribonuclease I (rhDNase). Enzyme treatment resulted in a dramatic decrease in DNA size, a redistribution of total DNA content from the pellet to supernatant, a significant decrease in that pellet volume and a decrease in elastic modulus. Sample elasticity, measured by a dynamic cone and plate viscometer, could be related to compaction assay results. These results suggest that the compaction assay may be a useful in vitro method for rapidly assessing the actions of enzymatic disruption of a complex biopolymer, such as that observed for the actions of rhDNase on purulent airway secretions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sputum
/
Cystic Fibrosis
/
Deoxyribonuclease I
Limits:
Humans
Language:
En
Journal:
Biomaterials
Year:
1995
Document type:
Article
Affiliation country:
United States