A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.
J Clin Microbiol
; 33(9): 2473-5, 1995 Sep.
Article
in En
| MEDLINE
| ID: mdl-7494051
ABSTRACT
A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Viral
/
Parvovirus
/
Parvoviridae Infections
Type of study:
Diagnostic_studies
/
Guideline
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
1995
Document type:
Article
Affiliation country:
United kingdom