Stoichiometry of a recombinant GABAA receptor deduced from mutation-induced rectification.
Neuroreport
; 5(3): 285-8, 1993 Dec 13.
Article
in En
| MEDLINE
| ID: mdl-7507726
ABSTRACT
Ligand-gated ion channels generally display a heterooligomeric subunit structure. The present report describes an electrophysiological method that provides criteria indicating the subunit stoichiometry of a recombinant GABAA receptor composed of alpha 3, beta 2 and gamma 2 subunits. Our results exclude the stoichiometries 3 alpha 1 beta 1 gamma, 1 alpha 3 beta 1 gamma, 1 alpha 1 beta 3 gamma and suggest that the possible subunit stoichiometries for this receptor are 2 alpha 1 beta 2 gamma, 2 alpha 2 beta 1 gamma or 1 alpha 2 beta 2 gamma, of which the alpha subunit composition 2 alpha 1 beta 2 gamma may be favoured. The method is based on the quantification of the outward rectification of the GABA-evoked current induced by point mutation of charged amino acids located near the ion channel pore.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, GABA-A
/
Mutation
Limits:
Animals
/
Humans
Language:
En
Journal:
Neuroreport
Journal subject:
NEUROLOGIA
Year:
1993
Document type:
Article
Affiliation country:
Switzerland