Cloning of FRK, a novel human intracellular SRC-like tyrosine kinase-encoding gene.
Gene
; 138(1-2): 247-51, 1994 Jan 28.
Article
in En
| MEDLINE
| ID: mdl-7510261
ABSTRACT
We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related kinase or FRK, is a novel member of the intracellular TYK gene family.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein-Tyrosine Kinases
/
Multigene Family
/
Genes, src
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Gene
Year:
1994
Document type:
Article