Accumulation of fibronectin in articular cartilage explants cultured with TGF beta 1 and fucoidan.
Arch Biochem Biophys
; 316(1): 452-60, 1995 Jan 10.
Article
in En
| MEDLINE
| ID: mdl-7530938
ABSTRACT
Fibronectin is a glycoprotein involved in cell matrix interactions. In osteoarthritis, fibronectin levels in the lesion cartilage are elevated up to 20-fold above control levels. In these experiments, explants of disease-free cartilage cultured in the presence of a combination of TGF beta 1 and the sulfated fucopolysaccharide, fucoidan, accumulated fibronectin at levels comparable to those found in osteoarthritic lesions. TGF beta 1 increased fibronectin synthesis, most of which was released to the medium. The addition of fucoidan favored retention of the newly synthesized fibronectin within the matrix. The fibronectin which accumulated as a result of these treatments was similar to the fibronectin in normal and osteoarthritic cartilage with respect to the ED-B+ alternative splice form. No change in the proteoglycan content of the cartilage explants with elevated fibronectin levels was detected.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoarthritis
/
Polysaccharides
/
Cartilage, Articular
/
Transforming Growth Factor beta
/
Fibronectins
Limits:
Animals
Language:
En
Journal:
Arch Biochem Biophys
Year:
1995
Document type:
Article