Polymorphonuclear leukocyte-platelet interaction: role of P-selectin in thromboxane B2 and leukotriene C4 cooperative synthesis.
Thromb Haemost
; 72(3): 450-6, 1994 Sep.
Article
in En
| MEDLINE
| ID: mdl-7531878
ABSTRACT
In PMN/platelet suspensions stimulated by fMLP giant mixed aggregates are formed and TxB2 and LTC4 are synthesized as the result of the cooperation in the arachidonic acid (AA) metabolism during cell/cell contact. PMN-derived cathepsin G induced the expression of P-selectin on platelet surface. GE12, an antibody against P-selectin, significantly reduced mixed cell aggregates. GE12 did not affect platelet aggregation induced by PMN-derived supernatants, indicating that the inhibitory effect of GE12 on mixed cell aggregation depends on inhibition of PMN/platelet adhesion. GE12 significantly reduced TxB2 and LTC4 production in PMN/platelet mixed cell suspensions stimulated by fMLP. As previously reported, synthesis of 3H-TxB2 in 3H-AA-labeled PMN/unlabeled platelets indicates that platelets utilize 3H-AA from PMN. 3H-LTC4 production in unlabeled PMN/3H-AA-labeled platelets indicates that bidirectional routes are utilized in this system for LTC4 synthesis. GE12 significantly reduced 3H-TxB2 and 3H-LTC4 synthesis. These results show that cathepsin G released by activated PMN induces the expression of P-selectin on platelet membrane this adhesive glycoprotein modulates cell-cell contact and transcellular metabolism of AA.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thromboxane B2
/
Blood Platelets
/
Platelet Membrane Glycoproteins
/
Leukotriene C4
/
Neutrophils
Limits:
Humans
Language:
En
Journal:
Thromb Haemost
Year:
1994
Document type:
Article
Affiliation country:
Italy