Enhancing the avidity of a human recombinant anti-HIV-1 monoclonal antibody through oligomerization.
J Pharm Sci
; 84(7): 866-70, 1995 Jul.
Article
in En
| MEDLINE
| ID: mdl-7562439
ABSTRACT
The oligomerization by chemical cross-linking of a recombinant human antiviral monoclonal antibody (MAb), r447-1, and its characterization are described. This MAb binds to an epitope residing in the hypervariable V3 region of the envelope protein (gp120/160) of HIV-1. A dimeric form of this MAb displays enhanced avidity and was found to be capable of neutralizing a greater variety of lymphoid cell culture-adapted HIV-1 variants and HIV-1 primary isolates than its monomeric form. The superior binding and breadth of reactivity of this antibody suggests it may have utility as a therapeutic and/or prophylactic agent, if it possesses an appropriate safety and immunogenicity profile.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
HIV-1
/
Antibodies, Monoclonal
Limits:
Humans
Language:
En
Journal:
J Pharm Sci
Year:
1995
Document type:
Article
Affiliation country:
United States