Regulation of Pax-3 expression in the dermomyotome and its role in muscle development.
Development
; 120(4): 957-71, 1994 Apr.
Article
in En
| MEDLINE
| ID: mdl-7600971
ABSTRACT
The segmented mesoderm in vertebrates gives rise to a variety of cell types in the embryo including the axial skeleton and muscle. A number of transcription factors containing a paired domain (Pax proteins) are expressed in the segmented mesoderm during embryogenesis. These include Pax-3 and a closely related gene, Pax-7, both of which are expressed in the segmental plate and in the dermomyotome. In this paper, we show that signals from the notochord pattern the expression of Pax-3, Pax-7 and Pax-9 in somites and the subsequent differentiation of cell types that arise from the somitic mesoderm. We directly assess the role of the Pax-3 gene in the differentiation of cell types derived from the dermomyotome by analyzing the development of muscle in splotch mouse embryos which lack a functional Pax-3 gene. A population of Pax-3-expressing cells derived from the dermomyotome that normally migrate into the limb are absent in homozygous splotch embryos and, as a result, limb muscles are lost. No abnormalities were detected in the trunk musculature of splotch embryos indicating that Pax-3 is necessary for the development of the limb but not trunk muscle.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Muscle, Skeletal
/
Gene Expression Regulation, Developmental
/
DNA-Binding Proteins
/
Mesoderm
Limits:
Animals
Language:
En
Journal:
Development
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
1994
Document type:
Article
Affiliation country:
United States