A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes.
Brain Res Mol Brain Res
; 30(1): 106-14, 1995 May.
Article
in En
| MEDLINE
| ID: mdl-7609631
ABSTRACT
We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5' E box not the 3' E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Regulatory Sequences, Nucleic Acid
/
Choline O-Acetyltransferase
/
Neurons
Limits:
Humans
Language:
En
Journal:
Brain Res Mol Brain Res
Journal subject:
BIOLOGIA MOLECULAR
/
CEREBRO
Year:
1995
Document type:
Article
Affiliation country:
United States
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS