Utility of cytokeratin 20 in identifying the origin of metastatic carcinomas in effusions.
Diagn Cytopathol
; 12(4): 303-8, 1995 Jun.
Article
in En
| MEDLINE
| ID: mdl-7656755
ABSTRACT
Using a commercially available monoclonal antibody (Ks20.1) and the avidin-biotin peroxidase method on cytospins and cell blocks, we analyzed cytokeratin (CK) 20 expression in 169 serous effusions. Cytoplasmic staining was observed in 44/151 malignant fluids. Colon, gastric, and pancreatic adenocarcinomas and mucinous ovarian tumors were most frequently positive. Single cases of transitional-cell and squamous cell carcinomas were reactive as well. Lung and breast cancers were mostly negative. Nonmucinous ovarian tumors were invariably unlabeled as were mesotheliomas and normal mesothelial cells. The study shows that CK 20 is valuable in distinguishing tumor cell origin in effusions. In particular, it identifies a set of carcinomas with the majority arising from the gastrointestinal tract, and represents a highly characteristic marker for colorectal cancer.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ascitic Fluid
/
Pleural Effusion, Malignant
/
Intermediate Filament Proteins
/
Neoplasms
Type of study:
Prognostic_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Diagn Cytopathol
Journal subject:
PATOLOGIA
Year:
1995
Document type:
Article
Affiliation country:
Italy