Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using direct injection fast anion-exchange chromatography and capillary gel electrophoresis.
J Chromatogr B Biomed Appl
; 662(2): 343-9, 1994 Dec 09.
Article
in En
| MEDLINE
| ID: mdl-7719488
ABSTRACT
The analysis of antisense phosphorothioate DNA (SODN) in human plasma via direct injection using anion-exchange high-performance liquid chromatography (AE-HPLC) is presented. The method relies on the ability to selectively extract phosphorothioate DNA from undigested serum, plasma and urine on anion-exchange resins. The automated HPLC method can analyze a sample every 5 min with a limit of detection of 50 ng/ml (ppb). The DNA was collected, desalted and analyzed by capillary gel electrophoresis. Due to the high resolving power of this technique, a qualitative assessment of enzymatic degradation of the antisense oligonucleotide can be made.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thionucleotides
/
Oligonucleotides, Antisense
Type of study:
Qualitative_research
Limits:
Humans
Language:
En
Journal:
J Chromatogr B Biomed Appl
Year:
1994
Document type:
Article