The regulatory effect of nucleoside diphosphate kinase on G-protein and G-protein mediated phospholipase C.
Chin Med Sci J
; 10(1): 25-9, 1995 Mar.
Article
in En
| MEDLINE
| ID: mdl-7780113
ABSTRACT
The effect of nucleoside diphosphate kinase (NDPK) on the activity of guanine nucleotide regulatory protein (G-protein) mediated phospholipase C (PLC) and on the [35S] GTPT tau S binding of G-protein was investigated in this work in order to demonstrate the mechanism behind the regulation of G-protein and its effector PLC by NDPK. The stimulation of PLC in turkey erythrocyte membrane by both GTP and GTP tau S indicated that the PLC stimulation was mediated by G-protein. NDPK alone stimulated PLC activity, as well as the stimulation in the presence of GTP and GDP, in a dose-dependent manner. However, NDPK inhibited GTP tau S-stimulated PLC. Furthermore, NDPK inhibited [35S]GTP tau S binding of purified Gi-protein in a non-competitive manner. A hypothesis implying an important role of direct interaction of G-protein and NDPK in the regulation of their functions is suggested and discussed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Type C Phospholipases
/
Nucleoside-Diphosphate Kinase
/
GTP-Binding Proteins
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Chin Med Sci J
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
1995
Document type:
Article