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Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
Moreno, M A; Pascual, C; Gibello, A; Ferrer, S; Bos, C J; Debets, A J; Suárez, G.
Affiliation
  • Moreno MA; Department de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
FEMS Microbiol Lett ; 124(1): 35-41, 1994 Nov 15.
Article in En | MEDLINE | ID: mdl-8001767
ABSTRACT
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
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Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / Aspergillus / Aspergillus nidulans / Transformation, Genetic / DNA Replication Language: En Journal: FEMS Microbiol Lett Year: 1994 Document type: Article Affiliation country: Spain
Search on Google
Add to My VHL
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / Aspergillus / Aspergillus nidulans / Transformation, Genetic / DNA Replication Language: En Journal: FEMS Microbiol Lett Year: 1994 Document type: Article Affiliation country: Spain