Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
FEMS Microbiol Lett
; 124(1): 35-41, 1994 Nov 15.
Article
in En
| MEDLINE
| ID: mdl-8001767
ABSTRACT
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Aspergillus
/
Aspergillus nidulans
/
Transformation, Genetic
/
DNA Replication
Language:
En
Journal:
FEMS Microbiol Lett
Year:
1994
Document type:
Article
Affiliation country:
Spain