A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova.
Gene
; 144(2): 155-61, 1994 Jul 08.
Article
in En
| MEDLINE
| ID: mdl-8039700
ABSTRACT
We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Nucleus
/
DNA, Protozoan
/
Oxytricha
/
DNA Polymerase II
Limits:
Animals
/
Humans
Language:
En
Journal:
Gene
Year:
1994
Document type:
Article