Expression of mRNA of murine bone-related proteins in ectopic bone induced by murine bone morphogenetic protein-4.
Cell Tissue Res
; 277(1): 27-32, 1994 Jul.
Article
in En
| MEDLINE
| ID: mdl-8055537
ABSTRACT
To determine whether a system of ectopic bone formation induced by osteosarcoma-derived bone-inducing substance (bone morphogenetic protein-4) can be used as a model of developing bone at the molecular level, we studied the expression of bone-related protein mRNAs in the process of ectopic bone formation using non-radioisotopic in situ hybridization. Osteonectin mRNA was detected in fibroblast-like cells, which are similar to periosteal cells from the early to middle stages of bone development. The proportion of osteonectin mRNA-expressing cells was greater than that of osteopontin mRNA-expressing cells in hypertrophic chondrocytes and osteoblast-like cells. In contrast, osteopontin mRNA was localized in a limited population of hypertrophic chondrocytes, a single layer of osteoblast-like cells adjacent to the bone trabeculae in the middle stage of bone formation, and in a limited subset of osteocytes in the late stage. A strong osteocalcin mRNA signal was detected in osteoblast-like cells from the middle to late stages and in a limited subset of osteocytes in the late stage of bone development. Since the sequential gene expression pattern of bone-related proteins in the present system is comparable to that in embryonic osteogenesis, this system may be useful as a model for studying gene expression in osteogenesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sialoglycoproteins
/
Bone and Bones
/
Bone Diseases
/
RNA, Messenger
/
Proteins
/
Gene Expression
/
Osteonectin
/
Choristoma
/
Growth Substances
Limits:
Animals
Language:
En
Journal:
Cell Tissue Res
Year:
1994
Document type:
Article
Affiliation country:
Japan