Mutational analysis of antistasin, an inhibitor of blood coagulation factor Xa derived from the Mexican leech Haementeria officinalis.
Thromb Res
; 75(1): 41-50, 1994 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-8073407
ABSTRACT
Antistasin is a Factor Xa inhibitor that is present in the salivary glands of the Mexican leech Haementeria officinalis. The antistasin protein consists of 119 amino acids, of which residues 1-55 (domain I) are 56% similar to residues 56-110 (domain II). Of the nine C-terminal amino acids (residues 111-119; domain III), four are positively charged. The reactive site for Factor Xa is located in domain I. In this study we assessed the role of separate domains and of individual amino acids in the reactive site for the inhibition of Factor Xa. A series of mutants was constructed and expressed in Chinese hamster ovary (CHO) cells. In vitro chromogenic assays for Factor Xa show that domain I is sufficient for inhibition of Factor Xa. Domains II and III neither contain any intrinsic Factor Xa inhibitory activity, nor contribute to the activity of domain I. Furthermore, domain II does not become a Factor Xa inhibitor by partially adaptating its sequence towards that of the reactive site in domain I. Mutation of the cysteine at position 33 is not crucial for Factor Xa inhibition, suggesting a relatively rigid reactive site loop structure.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Factor Xa Inhibitors
/
Invertebrate Hormones
/
Leeches
/
Anticoagulants
Limits:
Animals
Country/Region as subject:
Mexico
Language:
En
Journal:
Thromb Res
Year:
1994
Document type:
Article
Affiliation country:
Netherlands