Measurement of liver microsomal cytochrome p450 (CYP2D6) activity using [O-methyl-14C]dextromethorphan.

Rodrigues, A D; Kukulka, M J; Surber, B W; Thomas, S B; Uchic, J T; Rotert, G A; Michel, G; Thome-Kromer, B; Machinist, J M

Rodrigues, A D; Kukulka, M J; Surber, B W; Thomas, S B; Uchic, J T; Rotert, G A; Michel, G; Thome-Kromer, B; Machinist, J M.

Affiliation

Rodrigues AD; Drug Metabolism Department, Abbott Laboratories, Abbott Park, Illinois 60064.

Anal Biochem ; 219(2): 309-20, 1994 Jun.

Article in En | MEDLINE | ID: mdl-8080088

Subject(s)

Cytochrome P-450 Enzyme System/analysis; Dextromethorphan/metabolism; Microsomes, Liver/enzymology; Mixed Function Oxygenases/analysis; B-Lymphocytes; Carbon Radioisotopes; Cell Line; Chromatography, High Pressure Liquid/methods; Cytochrome P-450 CYP2D6; Cytochrome P-450 Enzyme System/biosynthesis; Cytochrome P-450 Enzyme System/metabolism; DNA, Complementary/metabolism; Dextromethorphan/chemical synthesis; Formaldehyde/analysis; Humans; Immunoassay; Isotope Labeling/methods; Kinetics; Magnetic Resonance Spectroscopy/methods; Mixed Function Oxygenases/biosynthesis; Mixed Function Oxygenases/metabolism; Radioisotope Dilution Technique; Substrate Specificity; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Microsomes, Liver / Cytochrome P-450 Enzyme System / Dextromethorphan / Mixed Function Oxygenases Limits: Humans Language: En Journal: Anal Biochem Year: 1994 Document type: Article Country of publication: United States

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