Detection of bovine herpesvirus 1 with various types of DNA probes.
Acta Vet Hung
; 41(1-2): 179-90, 1993.
Article
in En
| MEDLINE
| ID: mdl-8116497
ABSTRACT
The method of dot-blot hybridization on nitrocellulose filters by various types of DNA probes (ds recombinant plasmids, ss recombinant M13 phages and a 42bp synthetic oligonucleotide) was used for BHV-1 detection. The highest sensitivity was achieved with the 32P-pUR1 probe (1.8 kb random EcoRI-HindIII fragment inserted into pUC9) which detected the BHV-1 genome in 5 x 10(3) infected MDBK cells. Using the pUR1 probe, no cross hybridization was observed with other herpesviruses BHV-2, 3, 4, and Aujeszky's disease virus. The 32P-pUR1 probe detected BHV-1 in nasal swabs of calves as early as on day 1 after experimental infection. The maximum intensity of BHV-1 detection occurred on day 1-3. The 32P-pUR1 probe also detected BHV-1 in field samples of nasal swabs from cows and calves.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Probes
/
Cattle Diseases
/
Herpesviridae Infections
/
Herpesvirus 1, Bovine
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Acta Vet Hung
Year:
1993
Document type:
Article
Affiliation country:
Slovakia