Methotrexate-resistant variants of human dihydrofolate reductase. Effects of Phe31 substitutions.

Chunduru, S K; Cody, V; Luft, J R; Pangborn, W; Appleman, J R; Blakley, R L

Chunduru, S K; Cody, V; Luft, J R; Pangborn, W; Appleman, J R; Blakley, R L.

Affiliation

Chunduru SK; Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.

J Biol Chem ; 269(13): 9547-55, 1994 Apr 01.

Article in En | MEDLINE | ID: mdl-8144541

Subject(s)

Genetic Variation; Methotrexate/pharmacology; Phenylalanine; Tetrahydrofolate Dehydrogenase/chemistry; Tetrahydrofolate Dehydrogenase/metabolism; Amino Acid Sequence; Crystallography, X-Ray; Drug Resistance; Enzyme Stability; Genetic Therapy; Humans; Kinetics; Models, Structural; Models, Theoretical; Mutagenesis, Site-Directed; NADP/metabolism; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Tetrahydrofolate Dehydrogenase/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Phenylalanine / Tetrahydrofolate Dehydrogenase / Genetic Variation / Methotrexate Type of study: Prognostic_studies Limits: Humans Language: En Journal: J Biol Chem Year: 1994 Document type: Article Country of publication: United States

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