Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes.
J Gen Virol
; 75 ( Pt 4): 895-900, 1994 Apr.
Article
in En
| MEDLINE
| ID: mdl-8151302
ABSTRACT
Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endopeptidases
/
Vaccinia virus
/
Fusion Proteins, gag-pol
/
Infectious Anemia Virus, Equine
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
1994
Document type:
Article