Immobilized enzymes on chitosan columns: alpha-chymotrypsin and acid phosphatase.
Biotechnol Bioeng
; 18(10): 1445-54, 1976 Oct.
Article
in En
| MEDLINE
| ID: mdl-822899
ABSTRACT
alpha-Chymotrypsin and acid phosphatase have been immobilized on chitosan, a polyaminosaccharide, without using any intermediate reagent; the immobilized enzymes are active and their activity is much higher than for chitin-immobilized enzymes. The best pH conditions for operating chitosan columns have been determined and columns have been used to transform substrates in large amounts, with no decrease of activity or enzyme losses. Due to the nonconvalent interaction between chitosan and enzymes, the pure and active enzymes can be eventually recovered from the columns. The effects of metal ions, aldehydes, and salts are reported and discussed. Applications are foreseen in the food and biomedical sciences and industries.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acid Phosphatase
/
Chymotrypsin
Language:
En
Journal:
Biotechnol Bioeng
Year:
1976
Document type:
Article