Use of 10-propargyl-5,8-dideazafolate and directed mutagenesis to probe the catalytic mechanism of thymidylate synthase.

Spencer, H T; Villafranca, J E; Appleman, J R

Spencer, H T; Villafranca, J E; Appleman, J R.

Affiliation

Spencer HT; Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38101.

Adv Exp Med Biol ; 338: 575-8, 1993.

Article in En | MEDLINE | ID: mdl-8304183

Subject(s)

Folic Acid Antagonists/metabolism; Folic Acid/analogs & derivatives; Thymidylate Synthase/metabolism; Amino Acid Sequence; Binding Sites; Cysteine; Escherichia coli/enzymology; Escherichia coli/genetics; Folic Acid/metabolism; Folic Acid/pharmacology; Folic Acid Antagonists/pharmacology; Kinetics; Mutagenesis; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spectrometry, Fluorescence; Thymidylate Synthase/chemistry; Thymidylate Synthase/isolation & purification

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Collection: 01-internacional Database: MEDLINE Main subject: Thymidylate Synthase / Folic Acid / Folic Acid Antagonists Language: En Journal: Adv Exp Med Biol Year: 1993 Document type: Article

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