The atomic structure of Carnation Mottle Virus capsid protein.
FEBS Lett
; 338(3): 267-71, 1994 Feb 07.
Article
in En
| MEDLINE
| ID: mdl-8307192
ABSTRACT
The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Viruses
/
RNA Viruses
/
Capsid
/
Capsid Proteins
Type of study:
Prognostic_studies
Language:
En
Journal:
FEBS Lett
Year:
1994
Document type:
Article